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Kaninchen anti-Ratte IgG (Whole Molecule) Antikörper (PE)

FACS Polyclonal IgG PE F(ab')2 fragment

Produktnummer ABIN2622738

Kurzübersicht für Kaninchen anti-Ratte IgG (Whole Molecule) Antikörper (PE) (ABIN2622738)

Target

IgG

Fragment

F(ab')2 fragment

Klonalität

Polyklonal

Konjugat

PE

Applikation

Flow Cytometry (FACS)

Isotyp

IgG

Bindungsspezifität
2332

314

286

149

133

67

53

29

28

28

21

5

5

2

1

1

1

1

1

1

1

1

1
Whole Molecule
Reaktivität
640

572

554

407

361

232

190

155

134

125

111

88

86

85

83

62

48

31

28

18

16

10

5

4

4

4

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1

1

1
Ratte
Wirt
1556

1390

551

211

98

84

38

29

21

20

16

11

11
Kaninchen

Spezifität

Coupling to R-PE was followed by size exclusion chromatography to purify conjugate from unreacted R-PE and antibody. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit & Sheep Serum Proteins.

Aufreinigung

Immunoaffinity purified

Immunogen

Rat IgG whole molecule.

Type of Immunogen: Purified protein
Goat anti-Rat IgG antibody (Cy3) - Preadsorbed
WB, FACS, FLISA, FM, DB Polyclonal IgG Cy3

Goat anti-Rat IgG (Heavy Chain) antibody (TRITC)
ELISA, IHC, FACS Polyclonal IgG TRITC

Goat anti-Rat IgG (Heavy Chain) antibody
ELISA, IHC, WB, FACS Polyclonal IgG unconjugated

Goat anti-Rat IgG (Heavy Chain) antibody (FITC)
ELISA, IHC, FACS Polyclonal IgG FITC

Goat anti-Rat IgG (Heavy Chain) antibody (Biotin)
ELISA, IHC, WB, FACS Polyclonal IgG Biotin

Goat anti-Rat IgG (Heavy & Light Chain) antibody (beta-Gal) - Preadsorbed
ELISA, FACS, FLISA Polyclonal IgG beta-Gal

Goat anti-Rat IgG (Heavy & Light Chain) antibody (SPRD) - Preadsorbed
ELISA, FACS Polyclonal IgG SPRD F(ab')2 fragment

Rabbit anti-Mouse IgG antibody (Biotin)
ELISA, FACS, IHC (fro), IHC (p) Polyclonal IgG Biotin F(ab')2 fragment

Goat anti-Rat IgG (Heavy & Light Chain) antibody (FITC)
WB, FACS, FM, DB Polyclonal IgG FITC

Goat anti-Rat IgG (Fc Region) antibody (FITC)
FACS, FLISA, FM, DB Polyclonal IgG FITC

Applikationshinweise

Approved: Flo

Usage: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10 x10⁶ in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Kommentare

Target Species of Antibody: Rat

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Rekonstitution

deionized water

Konzentration

Lot specific

Buffer

0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA, 0.01 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung

Product is photosensitive and should be protected from light.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
Target

IgG

Substanzklasse

Antibody

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